Real Time PCR

 REAL TIME PCR SYSTEM5 CH (455-650nm) DAN 6 CH (510-715nm)

DESCRIPTION:
As a necessary choice for quantitative analysis of molecular biology, real-time PCR system has been widely used in various fields such as scientific research, clinical detection and diagnosis, quality and safety testing, and forensic applications. Up to 6 fluorescence detection channels allowing multiplex PCR. Effectively reduce multi-color cross talk and edge effect, no ROX correction required.
New optical scanning detection system Innovative scanning method and time-resolved signal separation technology Unique edge temperature compensation technology

SPECIFICATION:

ModelMODEL 5 CHANNELMODEL 6 CHANNEL
Sample capacity96
Reaction volume10-50 μl
Thermal cycle technologyPeltier
Temperature control system
Max. Heating/Cooling rate6.0° C/s
Heating temperature range4 – 100 °C
Temperature accuracy± 0.2°C
Temperature uniformity±0.2℃ @60℃ ,  ±0.3℃ @95℃
Temperature gradient setting range30–100°C
Temperature gradient difference setting range1 – 36°C
Detection system
Excitation light source5 monochrome high efficiency LEDs6 monochrome high efficiency LEDs
Detection devicePMT
Detection modeTime-resolved signal separating technology
Excitation/detection wavelength range455-650nm510-715nm
Fluorescent channels5 channels6 channels
Supported dyeFAM/SYBR Green, VIC/JOE/HEX/TET, ABY/NED/TAMRA/Cy3, JUN, ROX/Texas Red, Mustang Purple, Cy5/LIZ
SensitivitySingle copy gene
Resolution1.33 folds copy number difference can be distinguished in single-plex qPCR
Dynamic range10 orders of magnitude copies


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