Laboratory Filtration Methods
Filtration is to block suspended particles in liquid with membrane filters or porous materials, thereby separating solid and liquid substances in a heterogeneous mixture to perform separation and clarification. There are 3 common methods of laboratory filtration: by gravity, by vacuum and by pressure. Appropriate filtration method is to be selected according to the characteristics of the filtration sample, the removal efficiency of suspended particles, and the time spent on filtration.
Vacuum Filtration Principles Vacuum filtration is also known as negative pressure filtration, reduced pressure filtration or suction filtration. Vacuum filtration is to create negative pressure in the container underneath the membrane and accelerate liquid to pass through the membrane to separate solid from liquid.
Vacuum Filtration ApparatusVacuum filtration apparatus often includes equipment such as vacuum pump, safety trap, filter holder and receiver flask.
A. Vacuum PumpThe vacuum pump used in vacuum filtration is to extract the gas molecules in a specific space (usually a lab flask or bottle) to reach a certain pressure difference and accelerate filtration. Generally, a pump with vacuum of 685 mmHg is capable of performing most filtration experiments because 685 mmHg represents a pressure difference of 90%. Only few experiments may require pumps with higher vacuum. (* 1 atmosphere is 760 mmHg)How to select a proper vacuum pump?Select type of the pump by defining the sample whether it’s general water solution, organic solvent, or corrosive.Choose proper vacuum according to the state of the sample, such as the amount of suspended particles, the viscosity of the liquid, etc. Generally, more viscous or more impurity samples needs higher vacuum to obtain satisfactory results.Choose proper pump flow capacity based on the volume of the filter bottle. The larger the filter bottle is, the higher flow rate is required.Reasons to choose Rocker Oil-free Vacuum Pumps rather than general water pumps:Stable vacuum degree and decompression efficiency under different temperature.More environmental friendly for not needing additional water.Create no water pollution.Rocker 300 Vacuum Pump fits most laboratory experiments with Max. vacuum at 685 mmHg.Chart – Rocker Laboratory Filtration Pumps (Appendix I)B. Safety TrapThe safety trap is usually installed between the pump and the receiver bottle in a vacuum filtration system. Use of a safety trap is to prevent filtrate from flowing back into the pump due to improper operation, which may cause damage to the pump. A safety trap in laboratory filtration system can be a GL45 glass storage bottle, vacuum bottle or waste bottle. How do you protect pumps from liquid damage? Prote™ Filter Cartridge (YouTube video link)Read more: Chart – Rocker Lab Filtration Accessories (Appendix 2)C. Filter HolderFilter holders hold filter membranes and ensure liquid to pass through the membrane for filtration. Filter holders can be fixed on top of receiver bottles with silicone stoppers or spin lock mechanism. D. Receiver FlaskReceiver flasks are the containers connected to vacuum pump and collect filtrate, also known as suction flask or waste flask. They’re sometimes connected with a safety trap with silicone tubes. How to select proper filter holders and receiver flasks?Filter holders are to be chosen based on the volume of sample. Common size of filter holder includes 25 mm, 47 mm and 90 mm (in diameter). The larger the volume of sample, the larger size holder is suggested.Material of filter holder and receiver flasks should be selected according to chemical characteristics of sample solvent(s). For more information, please refer to chemical compatibility chart.
Vacuum Filtration Application(s)Aqueous solution filtration, such as suspended solids, microorganisms, etc.Clarification of cell culture media and buffers.Separation of chemical synthesis.Roughing filtration of industrial solvent.Appendix 1: Rocker Oil-free Vacuum PumpAll Rocker’ vacuuming pumps are oil-mist-free and maintenance-free.
For air
For liquid
Series
Rocker Vacuum Pump
Rocker C PTFE-coated Pump
Chemker Chemical Resistant Pump
Alligator Water Pump
Type
Oil-free
Drive
Piston
Diaphragm
Proper for Sample
Non-chemical
Organic solvent
Organic or corrosive solvent
Non-chemical
Feature
• Prote™ Filter Cartridge
• PTFE-coated, suitable for most chemicals.
• PTFE-made. High chemical resistance.
• Draws both air and liquid, direct drainage.
Appendix 3: Rocker Filter Holder(s) for Vacuum Filtration MF
LF
SF
VF
Pro
–
Connections
Magnetic
Spin-lock
Spin-lock
Clamp
Filter Area Dia.
47 mm
47 mm
47 mm
25 / 47 / 90 mm
Material
PPSU
PES
PES
SS316
Borosilicate glass
Autoclavable
Yes
Yes
Yes
Yes
Yes
Feature
• Single-hand operation.
• Fits most receiver flasks.
• Can be fitted on normal storage bottles.
• Can be flame sanitized.
• Selective filter of glass or stainless steel.
Application
• General filtration
• Suspended solid filtration
• Continuous filtration
• General filtration
• Suspended solid filtration
• Microbial detection
• Suspended solid filtration
• HPLC mobile phase pretreatment
• Organic solvent filtration
Vacuum Filtration Principles Vacuum filtration is also known as negative pressure filtration, reduced pressure filtration or suction filtration. Vacuum filtration is to create negative pressure in the container underneath the membrane and accelerate liquid to pass through the membrane to separate solid from liquid.
| For air | For liquid | ||
Series | Rocker Vacuum Pump | Rocker C PTFE-coated Pump | Chemker Chemical Resistant Pump | Alligator Water Pump |
Type | Oil-free | |||
Drive | Piston | Diaphragm | ||
Proper for Sample | Non-chemical | Organic solvent | Organic or corrosive solvent | Non-chemical |
Feature | • Prote™ Filter Cartridge | • PTFE-coated, suitable for most chemicals. | • PTFE-made. High chemical resistance. | • Draws both air and liquid, direct drainage. |
MF | LF | SF | VF | ||
Pro | – | ||||
Connections | Magnetic | Spin-lock | Spin-lock | Clamp | |
Filter Area Dia. | 47 mm | 47 mm | 47 mm | 25 / 47 / 90 mm | |
Material | PPSU | PES | PES | SS316 | Borosilicate glass |
Autoclavable | Yes | Yes | Yes | Yes | Yes |
Feature | • Single-hand operation. • Fits most receiver flasks. | • Can be fitted on normal storage bottles. | • Can be flame sanitized. | • Selective filter of glass or stainless steel. | |
Application | • General filtration • Suspended solid filtration • Continuous filtration | • General filtration • Suspended solid filtration | • Microbial detection • Suspended solid filtration | • HPLC mobile phase pretreatment • Organic solvent filtration |